β-globin gene framework variant: an African type?

β-Globin Gene Regulation and Nuclear Organisation

β-thalassemia for translating human β-globin gene and RNA interference of α-globin gene expression by lentivirus vectors

Objective: According to the characteristics of α/β-globin gene imbalance caused by α-globin gene accumulation in β-thalassemia, we designed two lentiviral vectors, that is, while we used the lentiviral to mediate full length human β-globin gene, we introduced the interference fragment of lentiviral α-globin gene to adjust the α/β chain ratio by increasing the expression of β-globin and decreasi...

transfection of human hematopoietic stem cells by a gene targeting construct containing the β-globin gene

background: replacment of cd133+ cell’s β globin gene by using a gene targeting construct containing the β globin gene and essential elements for homologous recombination. materials and methods: pfbggt was amplified, then digested using the nhei and xhoi restriction enzymes, and finally, a 13.3 kb band (naked dna) was extracted from the agarose gel. biological activity of positive and negative ...

Co-Inheritance of Beta & Delta-Globin Gene (HbYialousa) Mutations in an Iranian β-Thalassemia Carrier

Introduction: Beta-thalassemia is characterized by absence or reduced synthesis of the β-globin. Carriers of β-thalassemia, typically have microcytic hypochromic anemia and elevated hemoglobin HbA2 and normal HbF level. On the other hand carriers of severe alpha-thalassemia also have similar CBC parameters to that of β-thalassemia with normal HbA2 level. Co-presence of mutations in the β-globin...

β-globin gene cluster haplotypes in iranian patients with β-thalassemia

introduction: β-globin gene cluster haplotypes are useful in diagnosis of particular molecular defects in β-thalassemia, prenatal diagnosis of β-thalassemia, and elucidating population affinities. methods: β-globin gene cluster haplotypes were studied in 150 β-thalassemia minor and 52 healthy in-dividuals from the fars province of iran. dna was extracted from leukocytes of whole blood by phe-no...